FastQCFastQC Report
Thu 5 Sep 2024
wt_rep1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt_rep1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28283795
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGA1924640.6804744554258012No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG1874380.6627045628070773No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTAT1732640.6125910614187382No Hit
GCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTGCAGCGGG1510690.5341185650652609No Hit
GAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCC1333010.4712981408612246No Hit
GGTTTTTCTCCCCGCCGCTGCGCTGGTAAGCCTGTGTTTTGGTTCGCTATGGCCCGTACTAAGCAGACTGCTCGC1248590.44145066105874403No Hit
GTTTTAGGGAGGGAGAGCGGCCTGGGTCCTGGGTGTTGTGTGCGGAGCTGTGGCGTCGCGTGTGAGGCGCGTGC1140020.40306472310381264No Hit
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC1125980.3981007499170461No Hit
GGACTTTCCCGATCGCCAGGCAGGAGTTTCTCTCGGTGACTACTATCGCT1028280.36355800202907707No Hit
GTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT981760.34711042135611575No Hit
GTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTT897040.3171568737504992No Hit
GTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGG894270.3161775143682098No Hit
GGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGG835110.2952609435897835No Hit
GCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATAT823210.2910535873987207No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGG780350.275900033924019No Hit
GAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGA737970.26091618893433505No Hit
GAGAAGCTGTCTATCGGGCTCCAGCGGTCATGTCCGGCAGAGGAAAGGGC722810.2555562292825273No Hit
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG718470.25402178173049267No Hit
GGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGA689690.24384634381630896No Hit
GCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATC684520.24201844200893124No Hit
GCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGC673250.23803382820445418No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT629210.2224630747040841No Hit
GAGATTTTTGCGGCTATTTTCGTTGGTGTGTTGGTCATGTCTGGTCGCGGCAAAGGCGGAAAGGGACTGGGTAA623140.2203169694872983No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC616910.2181142947755066No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGAC609670.21555452512649026No Hit
GCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCAAGTGGAGGAAGAAGCGAATGCGC609230.21539895901522407No Hit
GGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA596070.21074611805098997No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA574860.20324712436927223No Hit
GATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACG555260.1963173612310512No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT553330.1956349916975427No Hit
GGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATC545150.1927428762653668No Hit
GAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACA536660.189741157436617No Hit
GCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACT526340.18609242500873732No Hit
GCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAAGACCCGG521560.1844024113454365No Hit
GCTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGGTGCG518210.18321798754375077No Hit
ATTTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGTCTAATACATGCATGTCGAGCGAAGGT486550.1720242987194611No Hit
GCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCC486290.17193237329007652No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN468870.1657733695213107No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC440020.1555731824530619No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC417890.1477489141750603No Hit
GGAAGTGAATCTAGCTCTGAAGGCATGGCGCGTACGAAGCAGACTGCTCG406490.14371833765589093No Hit
GGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGC392650.13882507633788183No Hit
GCTCTCCGCCAGACCGCCGCCGCGCCGCCATCATGGACACCAGCCGTGTG387520.1370113169042556No Hit
GACTGCGATAGGAATCATGTCTGGTCGCGGCAAAGGCGGAAAAGGCTTGG386400.1366153304392144No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGG380940.13468489642213854No Hit
GCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA376410.13308327259478442No Hit
GCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGCTT373410.13202259456342402No Hit
GAGACGCGGAGCTGGGAAAAGGGAGGCAGAGGAGGCGGAGGCAGAGGCAGAGGCAGAGCCCGGTGCCGAGACCA370210.13089120466330634No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA366200.1294734316947213No Hit
GCTTTCTCTCTCGCGCGCGGTGTGGTGGCAGCAGGCGCAGCCCAGCCTCG365910.12937089948502314No Hit
GCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGCGAGAAGAAAAAGGGCCGTTCTGCCATC365710.12930018761626577No Hit
GCCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG361990.12798494685737893No Hit
GCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGA356700.1261146179287468No Hit
GACAAAGCGTTGGGTGAGACTCCTCTTGCTCGTCATGTCTGGCCGCGGCA348620.1232578584309496No Hit
GTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAAC347060.1227063058546422No Hit
GCTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGTTTAATCCCTTTGTGACTTCCGACCG339120.11989904466497513No Hit
GCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAG333260.11782718691038456No Hit
GCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCA324070.11457797654098399No Hit
GTCCAGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGG319980.11313191882489602No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACG318820.11272178998610335No Hit
GCTCTACCGGCGGGATTTGATGGCGTGATGTCTCACAGAAAGTTCTCCGC317970.1124212645438846No Hit
GCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT312440.11046608137274365No Hit
GCTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC310810.10988977964237119No Hit
GAATCTTATTTTGTTGCGAGGTTCTGAGCGTTGTCTGTGTTTAACCTTGATTTCAGTCATGTCTGGTCGTGGCAA309740.10951147114451933No Hit
GCTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAGAAGAAGC306410.10833411852970933No Hit
GCCTTTTTGCTGTAGGCCCGGGTGGTTGCTGCCGAAATGGGCAAGTTCAT303430.1072805116852247No Hit
AGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCA298070.10538543360252753No Hit
GCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAAGTTCCAGC296810.10493994882935617No Hit
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTT295760.10456871151838004No Hit
GATCGGCGCTTTGCCACTTGTACCCGAGTTTTTGATTCTCAACATGTCCG287110.10151042319462435No Hit
GCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAGGA286100.1011533282573997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)